Z379794
Sigma
Antisense Technology: A Practical Approach
| ISBN-10: | 0-19-963583-8 |
| ISBN-13: | 978-0-19-963583-2 |
Description
| General description | Use of antisense oligonucleotides is a powerful technique for silencing specific genes, but it requires careful optimization and species-specific methods for success. Each chapter is written by an acknowledged expert with a record of success at the bench; each includes authoritative guidance, tips for success, and trouble-shooting advice. |
Properties
| publication info | C. Lichtenstein and W. Nellen, ed., Oxford University Press, 1998, 303 pp., soft cover |
Table Of Contents
| Table of Contents | 1. Nucleic acid structures 2. Evaluation of antisense effects 3. Selecting, preparing, and handling antisense oligodeoxyribonucleotides 4. In vitro RNAs 5. Catalytic antisense RNA based on hammerhead ribozymes 6. 2-5 A-Antisense chimaeras for targeted degradation of RNA 7. In vitro applications of antisense RNA and DNA 8. Antisense applications in Dictyostelium: a lower eukaryotic model system 9. Applications of antisense technology in plants 10. Antisense molecules and ribozymes: medical applications 11. Non-antisense effects of oligodeoxynucleotides 12. Antisense rescue 13. Shotgun antisense mutants 14. Detection of sense-antisense duplexes by structure-specific anti-RNA antibodies |
